منابع مشابه
Studies on the Active Site of Chymotrypsin.
as it rises in b to enter c above the midline, etc. It can be easily seen that a similar oscillation about the midline takes place in the rotating fluid annulus of Figure 4a and in the more general situation of Figure 4b. These oscillations can be made quite small by adjusting the density of the buffer to match the particle density and by speeding up the rate of buffer flow to shorten the perio...
متن کاملStudies on the Active Site of Clostripain
Clostripain is rapidly and selectively inactivated by the chloromethyl ketone derived from cr-N-tosyl-L-lysine (TLCK) with an apparent second order rate constant of 8.7 X lo4 M-I set-i at pH 6.5. Alkylation by TLCK occurs only with catalytically active enzyme, the rate of which is considerably retarded by the competitive inhibitors, oc-Ntosyl-L-homoarginine methyl ester and benzamidine. In cont...
متن کاملStudies on the active site of rhodanese.
The complete loss of enzymic activity on reaction with alkylating agents, aromatic nitro compounds, or aliphatic mercaptans indicates the importance of sulfhydryl groups for catalysis. Analyses during the course of inactivation with any of these reagents revealed the loss of one of the two -SH groups in the rhodanese monomer when inactivation was complete. Amino acid analysis of iodoacetate-ina...
متن کاملActive Site of alpha-Chymotrypsin Activation by Association-Desolvation.
High reactivity toward alpha-chymotrypsin is observed for derivatives of beta-arylpropionic acids of varied structure-L-alpha-acylamido compounds, D-cyclized compounds, and, now, L-glycolamide esters. Compensating enthalpy and entropy effects are observed which appear to be caused by changes in water of solvation. High reactivity with varied structure, and physical evidence, appear to rule out ...
متن کاملMechanism-Based Studies of the Active Site-Directed Inhibition and Activation of Enzyme Transketolase
Derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (PDC). The inhibition of transketolase, a thiamine requiring enzyme such as PDF, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (MNPB) is reported here. These studies indicate that the inhibitor binds to the enzyme at the active site. A two-step inhibition was observed, first th...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1964
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.51.6.1117